Brassinosleroids (BRs) are new groups of plant hormones with significant growth-promoting activity. Brassinolide (BL) is the most biologically active BR. BRs are considered as hormones, as they influence varied developmental processes like growth, germination of seeds, rhizogenesis, flowering and senescence. Senescence is regulated by phytohormones. This research shows that epibrassinolide (eBL) accelerates or delays senescence in wheat leaf segments. For this aim, epibrassinolide was applied exogenously into the excised wheat leaf segments. An increase in peroxidase activity and decrease in protease activity;, chlorophyll content (at 10and 0.1 mu M. eBL) were detected. However protein content decreased onlY at 10 mu M. eBL. Finally it was observed that eBR accelerated senescence specially at high concentration.