Leptin Gene Polymorphisms in Native Turkish Cattle Breeds

Oztabak K., Toker N. Y., ÜN C., Akis I., Mengi A., Karadag O., ...More

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.16, no.6, pp.921-924, 2010 (SCI-Expanded) identifier identifier


The regenerative medicine in animals is a rapidly growing field, especially when therapies with stem cells are applied. Currently, stem cells are used for treatment of orthopedic diseases occurring both in small and large animals. Non-steroidal anti-inflammatory drugs (NSAIDs) are routinely used and are often accompanied with adipose derived mesenchymal stem cells (ADMSCs) therapy. Therefore, it is reasonable to monitor morphology of cells and the proliferation status of canine and equine ADMSCs cultured with NSAIDs. We focused on the analysis of the above mentioned parameters. Morphology of investigated cells was monitored using an epifluorescence microscope and scanning electron microscope (SEM). Moreover, SEM analysis was carried out for determination of microvesicles secretion. Proliferation activity of AdMSCs was evaluated with a resazurin-based test. Our research showed that the lowest concentration of Flunixin meglumine (0.01 mg/ml) had a stimulating effect on canine AdMSCs proliferation, while the same concentration significantly slowed down equine stem cell growth. Interestingly, the 0.01 mg/ml concentration of Flunixin meglumine did not effect morphology of the investigated stem cells population. Thus, results obtained from multilevel research allow us to conclude that the lowest concentration of Flunixin meglumine may be accompanied with canine adipose derived mesenchymal stem cells in orthopedic treatment.

The aim of the study was to determine leptin gene polymorphisms in South Anatolian Red (SAR), East Anatolian Red (EAR) and Turkish Grey Cattle. In the study unrelated 40 SAR. 40 EAR and 40 Turkish Grey cattle were used. Target sites in leptin gene exon 2, exon 3 and intron 2 were amplified by polymerase chain reaction (PCR). The single nucleotide polymorphism (SNP) consisting site in exon 2, exon 3 and intron 2 were determined as a result of digestion with Kpn2l. HphI and Sau3Al restriction enzymes, respectively. The highest T allele frequency related with production traits for Kpn2l polymorphism was found for SAR cattle. For HphI polymorphism. T allele frequencies were detected clearly predominant. Within each breed for Sau3Al polymorphism B and C allele frequencies that effect production traits were found to be dramatically lower than A allele frequency. As a result we can suggest that there was no clearly difference that can create any advantage in terms of leptin gene SNPs among the three native Turkish cattle breeds.