Akademik Veri Yönetim Sistemi
Eurbee 10th Congress of Apidology, Tallinn, Estonya, 16 - 19 Eylül 2024, ss.222, (Özet Bildiri)
The insect antenna is a highly complex and multisensory organ that mediates chemosensory, mechanosensory, gustatory, and olfactory information. Pollination success is largely dependent on this sensory mechanism. However, the genetic mechanism of perception and odor discrimination in the antenna is still not fully understood, especially in bumblebees. In this study, we aimed to explore the differentiation of antennal chemosensory gene repertoires among different developmental stages (from larvae to adult) of B. terrestris and gene expression profiles among eugenol treated forager bumblebees. In the first step we tried to explore the correlation between gene expression levels at developmental stages with increasing and decreasing trends, espectively. Combined with the mRNA RNA-Seq and miRNA-seq data, the results of both positively and negatively correlated genes revealed that antennal transcriptomes are highly dynamic at different developmental stages. We also examined the expression profile of chemosensory and olfactory receptor genes and found that their expression was mainly positively correlated. 114 chemosensory-related genes were identifed including 68 ORs, 15 GRs, 19 IRs, nine OBPs, two CSPs, and two SNMPs in antennal tissue. Using adult antennae as a comparison, the number of differentially expressed genes were 87, 18, and 31 in larvae, early-stage pupae, and late- stage pupae, respectively. Secondly a set of bumble bee foragers were trained to give reflexive responses for eugenol and the other set was used as a control group which was not exposed to any sort of specific odor. The experiment group showed significant changes in the expressions of the OR2 , OR4 , OR47a, OR65a, OR49b and OR22c genes. We believe that our data will expand the understanding of antennal-specific functions and gene signatures by measuring global transcriptome dynamics.