Cratageus species are rich in phenols and flavonoids, which are active compounds that possess beneficial effects. This study aimed to identify the antioxidant, antimicrobial, DNA damage protection activities, and the capacities of acetylcholinesterase (AChE), thioredoxin reductase (ThxR), and alpha-glucosidase inhibition of the methanol extract of Cratageus microphylla. The plant extract inhibited the formation of free radicals in various assay systems, such as 2,-2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2'-diphenyl-1-picrylhydrazyl (DPPH), lipid peroxidation (anti-LPO) and ferric reducing antioxidant power (FRAP) assays. According to the findings, the extract inhibited the lipid peroxidation and neutralized stable DPPH, and ABTS cation radicals with EC50 values of 0.766 +/- 0.026 mg/mL, 0.207 +/- 0.009 mg/mLand 0.756 +/- 0.048 mg/mL, respectively. The extract also acted as a reductant as indicated by the FRAP value (2.841 +/- 0.096 mM Fe2+), thus exhibiting its potential as an antioxidant. Moderate AChE (4.94 +/- 0.29 mg/mL) and a-glucosidase (14.41 +/- 0.29 mg/mL) and significant ThxR (EC50= 0.61 +/- 0.06 mg/mL) inhibition activities were observed. DNA damage formation in in vitro assays was 51 % at 0.78 mu g/mL concentrations. Therefore, C. microphylla can be considered as a good source of antioxidant, antimicrobial, antidiabetic, anticholinesterase, and anticancer agents.