Akademik Veri Yönetim Sistemi
Forensic Science International, sa.380, ss.1-7, 2025 (Hakemli Dergi)
Effective DNA recovery from bone material is essential for applications in biomedical research, clinical diagnostics,
and forensic and archaeogenetic investigations. In this study, DNA isolation performance was evaluated
in an exploratory manner in human bone samples representing three preservation states: fresh,
cryopreserved, and ancient. All samples were processed using a unified bead mill homogenization and magnetic
bead–based extraction workflow in order to maintain procedural consistency. DNA quantity and purity were
evaluated by spectrophotometry, and amplifiability was assessed using nuclear and mitochondrial PCR assays as
well as representative STR profiling. Fresh and cryopreserved samples yielded higher DNA concentrations and
more consistent amplification than ancient specimens, in which recovery was primarily constrained by postmortem
degradation. PCR success demonstrated a clear dependence on amplicon length, with shorter mitochondrial
and nuclear targets amplifying more reliably across all sample types. Due to the limited sample size
and the use of a single individual per preservation group, the results are presented as qualitative observations
rather than as statistically generalizable conclusions. Within these constraints, the study demonstrates the
feasibility of using a standardized mechanical disruption and extraction workflow across bone samples of
differing preservation status and provides a methodological reference for future larger-scale studies involving
both modern and degraded skeletal material.