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“29th International Symposium on the Chemistry of Natural Products and the 9th International Conference on Biodiversity” (ISCNP-29 & ICOB-9), İzmir, Turkey, 24 - 27 September 2016, pp.6
Allium tuncelianum (Kollmann) Özhatay, B.Mathew & Şiraneci, known as Tunceli garlic, is an endemic species native to our country and distributed only in and near Tunceli. Tunceli garlic was firstly identified as a subspecies of A. macrochaetum Boiss. et Hausskn., however, in the following studies, it was determined to be a different species and named A. tuncelianum.
Because its chemical structure is similar to cultivated garlic (A. sativum), it is sold and used as commercial product by being collected from the mountains with the name “Rock garlic”, by local people. The plant is important in terms of being endemic and placed in the group of Vulnerable Plants in Red Data Book of Turkish Plants.
In our study, organosulfur compounds, categorized in volatile fatty acids, of the plant, Allium tuncelianum were extracted with solid phase micro extraction (SPME/PDMS) method and analysed via Gas Chromatography-Mass Spectroscopy . As a result of the analysis, diallyl disulphide compound was determined to be major compound at the rate of %50,51. Later on, cytotoxic activity of plant extracts on HeLa cells originating from human cervical carcinoma was determined and it was found that 72 h, 0,5 mg/ml concentration led 30 percent of the cells to apoptosis.
It is clearly advantageous to rehabilitate Allium tuncelianum plant by cultivating it, increase its production, and making the plant consumed on a global basis rather than its limited consumption by local people, as an alternative to Allium sativum (cultivated garlic) which has been consumed traditionally over centuries.
Key words: Allium tuncelianum, SPME, GC-MS, Cytotoxic Activity, HeLa Cells