Akademik Veri Yönetim Sistemi
JOURNAL OF ETHNOPHARMACOLOGY, cilt.357, 2026 (SCI-Expanded, Scopus)
Ethnopharmacological relevance: Lavandula stoechas L., native to the Mediterranean, has been traditionally used for its medicinal, aromatic, and decorative properties. Despite its known anti-inflammatory effect, integrated biological and computational assessments remain limited. Aim of the study: This study aimed to evaluate the anti-inflammatory potential and phytochemical profile of the hydro-methanolic extract of L. stoechas L. aerial parts. Materials and methods: Phytochemical analyses included total polyphenol, flavonoid, and flavonol content determination. The chemical profile was assessed via RP-HPLC-DAD, identifying key compounds such as coumaric acid, gallic acid, vanillic acid, and kaempferol. Anti-inflammatory activity was evaluated using an in vivo carrageenan-induced rat paw edema model, while in vitro assays included red blood cell membrane stabilization and protease inhibition. In silico analyses (ADME, toxicity, endocrine disruption, and molecular docking) were performed to assess pharmacological properties. Results: The extract exhibited high polyphenol (156.08 +/- 0.68 mg GAE/g) and flavonoid (255.5 +/- 1 mg QE/g) contents. RP-HPLC confirmed the presence of key phenolics. At 500 mg/kg, the extract significantly reduced paw edema (43.3 % at 120 min), outperforming indomethacin at times. In vitro, the extract showed notable membrane stabilization (28.83 %) and protease inhibition (98.66 %). In silico, kaempferol had the strongest COX-2 binding (-8.0 kcal/mol) with favorable ADME and low toxicity profiles for all compounds. Conclusion: The phenolic-rich L. stoechas L. extract demonstrates strong anti-inflammatory activity, likely due to synergistic bioactive interactions. Its safety and efficacy support further exploration as a natural alternative to conventional anti-inflammatory therapies.