Molecular Monitoring in Stem Cell Therapy: Emerging Technologies in Chimerism Analysis
Stem Cell Treatment Strategies and Advances, Dr. Mani T. Valarmathi, Editör, IntechOpen, London, ss.1-13, 2026
- Yayın Türü: Kitapta Bölüm / Mesleki Kitap
- Basım Tarihi: 2026
- Doi Numarası: 10.5772/intechopen.1015775
- Yayınevi: IntechOpen
- Basıldığı Şehir: London
- Sayfa Sayıları: ss.1-13
- Editörler: Dr. Mani T. Valarmathi, Editör
- İstanbul Üniversitesi Adresli: Evet
Özet
Stem cell transplantation has become a cornerstone in the treatment of hematologic malignancies and various genetic disorders, with posttransplant monitoring playing a vital role in predicting clinical outcomes. Chimerism analysis enables the assessment of donor-recipient cell dynamics and serves as an essential indicator for engraftment success, risk of relapse, and graft-versus-host disease (GVHD). Recent technological advances have significantly enhanced the sensitivity, specificity, and clinical applicability of chimerism detection. Among these, next-generation sequencing (NGS) has transformed molecular monitoring by providing high-resolution characterization of mixed chimerism, while digital droplet polymerase chain reaction (ddPCR) offers robust quantification of low-level residual recipient cells. In addition, emerging single-cell and epigenetic profiling approaches hold promising potential for identifying early immune reconstitution patterns and relapse-associated minimal residual disease (MRD). This abstract highlights the evolution of chimerism assays from conventional short tandem repeat (STR)-based techniques toward precision-driven molecular platforms and emphasizes the importance of integrating novel analytical tools into clinical transplantation practice. Continuous innovation in chimerism monitoring will enable earlier intervention and improved long-term outcomes for stem cell transplant recipients.