Chemometrics Evaluation of Phytochemicals and Antioxidant Activities of the Extracts of Chaerophyllum Bulbosum Roots and Aerial Parts

Tel-Cayan G., Deveci E., Cayan F., Molo Z., Duru M. E., YEŞİL CANTÜRK Y.

ANALYTICAL LETTERS, cilt.55, sa.2, ss.327-342, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 55 Sayı: 2
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1080/00032719.2021.1930026
  • Dergi Adı: ANALYTICAL LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.327-342
  • Anahtar Kelimeler: Antioxidant activity, C, bulbosum, chemometric analysis, high-performance liquid chromatography, diode array detection (HPLC-DAD), phytochemical compounds, spectrophotometry, MEDICINAL-PLANTS, ANTIMICROBIAL ACTIVITIES, OXIDATIVE STRESS, ROSMARINIC ACID, MYRICETIN, PROFILE, FOOD
  • İstanbul Üniversitesi Adresli: Evet

Özet

The objective of this study was provide the first report to assess the high-performance liquid chromatography-diode array detection (HPLC-DAD) identification of phytochemicals, the spectrophotometric determination of total phenolics and flavonoids, and antioxidant activities of the root and aerial extracts of Chaerophyllum bulbosum. HPLC-DAD identified the main phytochemical to be rosmarinic acid in the roots in the acetone (CBRA) (13.98 +/- 0.12 mg/g) and the aerial water (CBAW) extract; myricetin (8.30 +/- 0.22 mg/g) in the root methanol extract (CBRM); trans-aconitic acid (6.46 +/- 0.49 mg/g) in the root water extract (CBRW) and fumaric acid in the aerial methanol (CBAM) (17.43 +/- 0.87 mg/g) and water CBAW (18.96 +/- 0.81 mg/g) extracts. The concentrations of total phenolics and flavonoids were 2.00 +/- 0.14-17.79 +/- 0.99 mu g PEs/mg extract and 2.90 +/- 0.00-27.64 +/- 0.37 mu g QEs/mg extract, respectively. The highest antioxidant activity was found in the root acetone extract (CBRA) using the beta-carotene-linoleic (IC50: 172.31 +/- 0.16 mu g/mL) and CUPRAC (A(0.50): 126.00 +/- 0.24 mu g/mL) assays and in the root water extract (CBRW) for DPPH center dot (IC50: 59.51 +/- 0.21 mu g/mL) and metal chelating (IC50: 65.91 +/- 0.36 mu g/mL) assays in the aerial water extract (CBAW) using the ABTS(center dot+) (IC50: 59.51 +/- 0.21 mu g/mL) assay. The relationship between the phytochemicals and antioxidant activities of the extracts was chemometrically determined by principal component analysis (PCA) and hierarchical cluster analysis (HCA).