Acrylamide disrupts the steroidogenic pathway in Leydig cells: possible mechanism of action


Yildizbayrak N. , Erkan M.

TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY, vol.100, no.2, pp.235-246, 2018 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 100 Issue: 2
  • Publication Date: 2018
  • Doi Number: 10.1080/02772248.2018.1458231
  • Title of Journal : TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY
  • Page Numbers: pp.235-246

Abstract

Acrylamide-treated Leydig cells were tested for cytotoxicity, testosterone secretion, 3,5-cyclic adenosine monophosphate production, and gene and protein expression of steroidogenic genes and transcription factors. Reverse-transcriptional real-time polymerase chain reaction and western blot analysis revealed that acrylamide disrupts mRNA and protein expression of steroidogenic markers, including luteinizing hormone receptor, steroidogenic acute regulatory protein, cholesterol side-chain cleavage enzyme, 3-hydroxy dehydrogenase, and 17-hydroxy dehydrogenase. Further, transcription levels of the key regulator transcription factors, steroidogenic factor-1, GATA binding protein-4, and nerve growth factor IB, were evaluated. Acrylamide induced cytotoxicity and decreased testosterone and 3,5-cyclic adenosine monophosphate secretion by altering the rate-limiting steps in Leydig cell steroidogenesis.