Acta Pharmaceutica Turcica, vol.17, no.1, pp.1-5, 2000 (National Refreed University Journal)
The purpose of this study was to develop analysis methods for the spectrophotometric determina tion of amlodipine besylate and aspartame that have primary aliphatic amine groups after derivatization with NQS which form coloured and stable derivatives with substances having those groups, and to apply the developed methods to the determination of these active substances in pharrnaceutical preparations. The reaction for amlodipine besylate was completed at pH 8.5, in aqueous solution at 50°C within 20 min, with a reagent/amine mole ratio of 6. The reaction for as partame was completed at pH 8.5 and 40'C in 20 mins, with a reagent/amine mole ratio of 10. The absorbances of the derivatives formed were measured at 462 nm and 444 nm for amlodipine besyl ate and aspartame respectively. The Lambert Beer 's law was found to be valid at the concentration ranges of 10-80 µg/mL and 10-60 µglmL, for the derivatives respectively. Results obtained from the developed methods applied to the determination of amlodipine besylate and aspartame in tablets were compared statiscally with the results obtained by UV-spectrophotometric and non aqueous titration.