Spectrophotometric Determination of Amlodipine Besylate and Aspartame in Tablets


İskender G., SAĞIRLI A. O.

Acta Pharmaceutica Turcica, vol.17, no.1, pp.1-5, 2000 (National Refreed University Journal)

  • Publication Type: Article / Article
  • Volume: 17 Issue: 1
  • Publication Date: 2000
  • Title of Journal : Acta Pharmaceutica Turcica
  • Page Numbers: pp.1-5

Abstract

The purpose of this study was to develop analy­sis methods for the spectrophotometric determina­ tion of amlodipine besylate and  aspartame that have primary aliphatic amine groups after de­rivatization with NQS which form coloured and stable derivatives with substances having those groups, and to apply the developed methods to the determination of these active substances in phar­rnaceutical preparations. The reaction for amlodipine besylate was completed at pH 8.5, in aqueous solution at 50°C within 20 min, with a rea­gent/amine mole ratio of 6. The reaction for as­ partame was completed at pH 8.5 and 40'C in 20 mins, with a reagent/amine mole ratio of 10. The absorbances of the derivatives formed were measured at 462 nm and 444 nm for amlodipine besyl­ ate and aspartame respectively. The Lambert­ Beer 's law was found to be valid at the concentration ranges of 10-80 µg/mL and 10-60 µglmL, for the derivatives respectively. Results  obtained from the developed methods applied to the deter­mination of amlodipine besylate and aspartame in tablets were compared statiscally with the results obtained by UV-spectrophotometric and non­ aqueous titration.