The purpose of this study was to evaluate the effect of toothbrushing on the surface roughness and microhardness of Admira and Definite following acid conditioning. Half of the samples were either used as controls or brushed for 10 minutes using an electric toothbrush with slurry. The remaining specimens were stored in orthophosphoric acid with a pH of 1.9. for 24 hours and then half were brushed. The surface roughness of Admira and Definite was not significantly affected by storage under acidic conditions. However, the specimens of both materials in the control groups and the ones tested after acidic storage were found to be significantly smoother than the specimens subjected to toothbrushing alone and brushing following acid conditioning. For both materials, significantly higher values of microhardness were obtained after toothbrushing following acid conditioning than toothbrushing alone. Therefore, brushing following acid conditioning significantly affects the surfaces of the ormocers.