Investigation of methylation and expression levels of the guanine nucleotidebinding protein gamma-7 (GNG7) gene in oral squamous cell carcinoma

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Bedikyan N., Ulusan M., Olgaç N. V., Demokan S.

East Black Sea Journal of Health Sciences, cilt.2, sa.1, ss.45, 2023 (Hakemli Dergi)

  • Yayın Türü: Makale / Özet
  • Cilt numarası: 2 Sayı: 1
  • Basım Tarihi: 2023
  • Dergi Adı: East Black Sea Journal of Health Sciences
  • Derginin Tarandığı İndeksler: Index Copernicus
  • Sayfa Sayıları: ss.45
  • İstanbul Üniversitesi Adresli: Evet

Özet

Introduction and Aim: Oral squamous cell carcinoma (OSCC) is responsible for more than 91% of all malignancies in oral cavity. Epigenetic alterations and other environmental factors can cause changes in gene expression of OSCC pathogenesis. DNA methylation is just one of many epigenetic alterations. Herein, in our study we focused on predictive biomarker potential of guanine nucleotidebinding protein γ-7 (GNG7) gene methylation. The clinical significance of GNG7 methylation and the association with oral carcinogenesis is still remain unknown. Materials and Methods: DNA and RNA samples of tissues and body fluids obtained from OSCC patients and healthy individuals were used to examine the methylation and expression levels of the GNG7 gene by using QMSP/QRT-PCR methods, respectively. All results were compared with clinicopathological and demographical parameters. Results: GNG7 gene hypermethylation was observed in 18% of patients. It was observed decreased expression levels in 48% and increased expression levels in 32% OSCC patients. There was a statistical significance was found between the classification of retromolar trigone with tongue, floor of the mouth and decreased expression levels. Decreased expression levels of GNG7 gene in tumor, matched-normal tissues were downregulated in patients than healthy individuals and GNG7 gene expression levels in patients and healthy individuals’ serum were seen abundant significance. Conclusion: The GNG7 gene promoter hypermethylation depends on loss of expression in OSCC patients. The result of loss of expression due to the existence of tumor hypermethylation compared to healthy people suggests that there is a specific subgroup of OSCC patients for the GNG7 gene in Turkish population. Keywords: Oral squamous cell carcinoma; GNG7 gene; Epigenetics; Expression; Methylation This study was supported by Scientific Research Projects Coordination Unit of Istanbul University (Project number: IU-BAP-TYL-2019-35412).