Akademik Veri Yönetim Sistemi
17th Congress of the European Geriatric Medicine Society, Athens, Yunanistan, 11 - 13 Ekim 2021, ss.58, (Özet Bildiri)
Introduction: Healthy life is closely related with the homeostatic relationship established between gut microbiota (GM) and the human body. In this study, our aim is to investigate the age-related changes in human GM in Istanbul City of Turkey, using next-generation sequencing based on 16S ribosomal RNA amplicon sequencing strategy. Methods: 163 individuals were enrolled into the study (male/female: 69/94, 48 nursing home residents [NH] and 115 community dwelling individuals [CD]). The V3–V4 16S rRNA hypervariable regions of human GM samples were sequenced using the Illumina MiSeq system by the paired-end sequencing method. Bioinformatics analysis was performed by the QIIME 2 software. Sub-programs of QIIME 2 was used for statistical analysis. Results: Firmicutes ratio were decreased, while Proteobacteria and Verrucomicrobiota were increased with ageing in CD elderly. Actinobacteria were more abundant in NH group. Firmicutes/Bacteroidota ratio was 32.8–45.0 in NH group, 18.2–35.1 in CD group (65–94 years of age) and 9.6 in CD (centenarians). Akkermansia showed strong correlation with ageing. Centeranians had decreased Firmicutes and increased Proteobacteria and Verrucomicrobiota. The predominant Bifidobacterium species of our centenarians was a previously unidentified species (median 1.0%, mean 2.96%). Christensenellaceae R-7 group relative abundance was highest in centenarian (median: 3.20%). Key conclusions: As well as we know, this is the first study in Turkey that investigated possible relationship of human gut microbiota and longevity using next-generation sequencing. Although it has some limitations, our study presents the first findings in defining native to our country-specific gut microbiota compositions for various age groups.