ANALYZING OF ANTHOCYANIN ACCUMULATION AND GROWTH RATIOS OF Black carrot SSP. CALLUS UNDER THE SALICYLIC ACID AND SALINITY STRESSES


Odabaşı B., Şebikbay S., Pulat E., Çakır Ö.

III. International Agricultural, Biological & Life Science Conference, Edirne, Turkey, 29 - 31 August 2022, pp.536

  • Publication Type: Conference Paper / Summary Text
  • City: Edirne
  • Country: Turkey
  • Page Numbers: pp.536
  • Istanbul University Affiliated: Yes

Abstract

Carrot (Daucus carota), a member of the Apiaceae family, is rich in antioxidants. Especially

purple carrot plants produce a secondary metabolite from the flavonoid family called

anthocyanin. These secondary metabolites are known to have significant antioxidant activity.

Salinity is an abiotic stress that threatens crop yield, including disturbing osmotic equilibrium

and generating reactive oxygen species in plant cells. Exposure to salinity is known to induce

or stimulate the production of plant seconder metabolites. Salicylic acid (SA, 2-hydroxybenzoic

acid), one of the plant growth regulators, is used in the stimulation of many signaling pathways

involved in various plant defense responses. The aim of this study is application of different

elicitors to increase anthocyanin accumulation by activation of plantdefense pathways. In this

regard; seeds of ''Daucus carota ssp. (purple carrot)'' taken from the ‘’Republic of Türkiye

Ministry of Agriculture and Forestry Atatürk Horticultural Central Research Institute’’ were


used and studies were carried out to obtain callus culture. First of all, the seeds were surface-

sterilized and MS medium was prepared for seeds to plant and germinate. Explants which were


obtained from hypocotyl of germinating plants were used to establish callus cultures in MS

medium containing 50 uL of BAP (6-Benzylaminopurine) and 500 uL of 2,4-D (2,4-

dichlorophenoxyacetic acid). 1 month-old calli were exposed to different concentrations of

saliyclic acid and sodium chloride, then the changes in anthocyanin amounts and growth ratios

were calculated through 20 days and samples were taken in every 5 days. Anthocyanin amounts

in the calli were analyzed by UV-visible spectrophotometer method and fresh weights were

measured. Results of growth measurements has showed that, when growth rate of control group

was increased 5.7 fold at the end of 20 days, in 50 mM NaCl it was 6.04 fold and 3.6 fold in 50

uM SA, respectively. The outcomes of this study might serve to understand better the

relationship between the anthocyanin production and salicylic acid, salinity.