The composition of fatty acids and sterols was investigated in different organs of Rapana venosa. The isolated lipids were saponified then esterified, and fatty acid esters were analyzed by gas chromatography (GC). The total fatty acid content in the hepatopancreas was > right massive gland salivary > gonad > flesh. In the flesh, the ratio of saturated to unsaturated fatty acids was 0.50. The highest amounts of fatty acids in the flesh were stearic acid in the saturated fatty acids, and n-3 and n-6 acids in the polyunsaturated fatty acids at 0.30, 0.32, and 0.38 mu g/mg, respectively. The sterols isolated from R. venosa were analyzed by gas chromatography/mass spectrometry (GC/MS). Two sterols were identified in the flesh and nine sterols in the whole organs.