Influence of biological assay conditions on stability assessment of radiometal-labelled peptides exemplified using a 177Lu-DOTA-minigastrin derivative

Ocak M., Helbok A., von Guggenberg E., Ozsoy Y., Kabasakal L., Kremser L., ...Daha Fazla

Nuclear Medicine and Biology, cilt.38, sa.2, ss.171-179, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 2
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.nucmedbio.2010.07.009
  • Dergi Adı: Nuclear Medicine and Biology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.171-179
  • Anahtar Kelimeler: Radiopeptides, In vitro stability, In vivo stability, Radiometabolites, IN-VIVO EVALUATION, INITIAL CLINICAL-EVALUATION, RADIOLABELED PEPTIDES, POSITIVE TUMORS, RECEPTOR, VITRO, ANALOGS, MINIGASTRIN, SCINTIGRAPHY, STRATEGIES
  • İstanbul Üniversitesi Adresli: Evet

Özet

Introduction: Lack of correlation between in vitro and in vivo stability is a general problem for the development of radiopeptides especially in the case of minigastrin derivatives for therapeutic applications. In this study, we compared the influence of experimental conditions on radiopeptide stability results in vitro using a model Minigastrin (MG) analogue labelled with Lu-177. Additionally, we attempted to characterize the main serum enzymatic cleavage sites by matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) analysis.