Peripheral blood expression levels of inflammasome complex components in two different focal epilepsy syndromes.


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Ulusoy C. , Vanlı-Yavuz E., Şanlı E., Timirci-Kahraman Ö., Yılmaz V. , Bebek N. , ...Daha Fazla

Journal of neuroimmunology, cilt.347, ss.577343, 2020 (SCI Expanded İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 347
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.jneuroim.2020.577343
  • Dergi Adı: Journal of neuroimmunology
  • Sayfa Sayıları: ss.577343

Özet

Although the role of inflammation in epilepsy pathogenesis has been extensively investigated, the

inflammasome complex, a key component of neuroinflammation, has been understudied in epilepsy patients.

Methods: To better understand the involvement of this system in epilepsy, levels of inflammasome complex

components (NLRP1, NLRP3, CASP1, ASC), end-products of inflammasome complex activity [IL-1β, IL-18, nitric

oxide synthase (NOS) isoforms] and other inflammatory factors (NFκB, IL-6, TNF-α) were measured in peripheral

blood of patients with focal epilepsy of unknown cause (FEoUC) (n = 47), mesial temporal lobe epilepsy

with hippocampal sclerosis (MTLE-HS) (n = 35) and healthy controls using real time qPCR and/or ELISA.

Results: Inflammasome complex associated factors were either downregulated or unchanged in epilepsy patients.

Likewise, flow cytometry studies failed to show an increase in ratios of NLRP3-expressing CD3+ and CD14+

peripheral blood mononuclear cells (PBMC) in epileptic patients. Anti-neuronal antibody positive epilepsy patients

showed increased NLRP1 and neuronal NOS mRNA expression levels, whereas patients under poly-therapy

showed reduced serum inflammasome levels. FEoUC patients demonstrated increased PBMC NFκB mRNA expression

levels and serum IL-1β and IL-6 levels. Both MTLE-HS and FEoUC patients displayed higher ratios of

NFκB-expressing CD14+ PBMC than healthy controls.

Conclusions: Although previous clinical studies have implicated increased inflammasome complex expression

levels in epilepsy, our results indicate suppressed inflammasome complex activity in the peripheral blood of focal

epilepsy patients. Alternatively, the IL-6-NFκB signaling pathway, appears to be activated in focal epilepsy,

suggesting that factors of this pathway might be targeted for future theranostic applications.