Inhibition of skin tumorigenesis by Brassica Oleracea Var Capitata

ISBIR T., YAYLIM İ., KOYUNCU H., AGACHAN B., ZEYBEK U., AYDIN M., ...Daha Fazla

Journal of B.U.ON., cilt.4, sa.4, ss.411-415, 1999 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 4 Sayı: 4
  • Basım Tarihi: 1999
  • Dergi Adı: Journal of B.U.ON.
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.411-415
  • Anahtar Kelimeler: Brassica oleracea, CD-1 mice, DMBA, Skin tumorigenesis, TPA
  • İstanbul Üniversitesi Adresli: Evet

Özet

Purpose: To investigate possible anti-tumor effects of the Brassica oleracea var. capitata extract (brassica) on skin tumor initiation and promotion in female CD-1 mice. Materials and methods: Fifty kg of brassica leaves were extracted by using petrol ether and ethyl alcohol. Eighty CD-1 mice 7-9 week-old were divided in 4 groups of 20 animals each and, in the two-stage skin carcinogenesis model, the protocols used to elicit tumors were a single topical application of 200 nmol of the initiator 7,12-dimethyl-benz(a)anthracene (DMBA) to the backs of mice, followed 1 week later by promotion with 10 nmol of 12-O-tetradecanoyl-phorbol-13 acetate (TPA), twice weekly for 30 weeks in the 1st group. In addition to this regimen, 0.1 g/L brassica extract in drinking water, one week prior to the initiating dose of DMBA, was applied in the 2nd (treatment) group. The 3rd group received a single topical application of DBMA and topical acetone twice weekly for 30 weeks. The 4th (control) group animals received a single or multiple applications of acetone. The antitumor effect of brassica extract was evaluated by studying the T and B lymphocytes counts. Results: All skin tumors were papillomas. In the 1st group of mice a 100% tumor incidence was reached by the 24th week of the study with an average of 5.1 skin tumors per mouse. In the 2nd (treatment) group an 83% tumors per mouse. In the 2nd (treatment) group an 83% mouse was observed. Tumor incidence and tumor multiplicity considerably favored the treatment group (p < 0.001 for both variables). In the treatment group, the T cell count was significantly higher compared to the 1st group (p < 0.02), and the B cell count was significantly lower (p < 0.01). Conclusion: These results demonstrate that some of the antitumor protective effects of brassica extract on mouse skin carcinogenesis induced by the DMBA/TPA model may result from its enhancement of the T cells responses as well as of antioxidant protective systems.