Application of next-generation sequencing methods for microbial monitoring of anaerobic digestion of lignocellulosic biomass

Bozan M., Akyol C., İnce O., Aydin S., Ince B.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, cilt.101, sa.18, ss.6849-6864, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 101 Sayı: 18
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1007/s00253-017-8438-7
  • Dergi Adı: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.6849-6864
  • Anahtar Kelimeler: Anaerobic digestion, Biogas, Lignocellulose, Microbial community, Next-generation sequencing, COMPLETE GENOME SEQUENCE, BIOGAS-PRODUCING COMMUNITY, METAGENOMIC ANALYSIS, BACTERIAL EPIGENOMICS, BUFFALO RUMEN, WHEAT-STRAW, DYNAMICS, MANURE, PLANT, DIVERSITY
  • İstanbul Üniversitesi Adresli: Hayır

Özet

The anaerobic digestion of lignocellulosic wastes is considered an efficient method for managing the world's energy shortages and resolving contemporary environmental problems. However, the recalcitrance of lignocellulosic biomass represents a barrier to maximizing biogas production. The purpose of this review is to examine the extent to which sequencing methods can be employed to monitor such biofuel conversion processes. From a microbial perspective, we present a detailed insight into anaerobic digesters that utilize lignocellulosic biomass and discuss some benefits and disadvantages associated with the microbial sequencing techniques that are typically applied. We further evaluate the extent to which a hybrid approach incorporating a variation of existing methods can be utilized to develop a more in-depth understanding of microbial communities. It is hoped that this deeper knowledge will enhance the reliability and extent of research findings with the end objective of improving the stability of anaerobic digesters that manage lignocellulosic biomass.