Research Information System
2015 IADR/AADR/CADR General Session & Exhibition (March 11-14, 2015)., Boston, United States Of America, 11 - 14 March 2015
A new modified SLA and SLA Active surface treatment for dental implants
Erdilek D, Buğur T, Taş A, Öksüzömer F, Güven Y.
The surface topography plays an important role in the osseointegration of titanium implants and the surface modifications are key factors for the initial interactions between the implant and bone tissue. Osteoblast cell spreading is important for the long-term success of dental implants.
The aim of this in vitro study was to evaluate the adhesion and spreading of Saos-2 osteoblasts to chemically modified hydrophilic SLA Active surfaces compared with standard SLA implants.
Materials and Methods: Titanium implant surfaces were modified by conditioning with different sandblasting and acid etching treatments. The surface topography was examined by scanning electron microscopy (SEM). Saos-2 cells were seeded on the sample surfaces. After 1 hour ,5 hrs and 24 hrs, the total number of the cells were determined by using Hoescht dye; the cells were stained with α-actin antibody and the ratio of the spread cells was observed under a fluorescence microscope. Cell morphology of Saos-2 cells was examined after one week cultivation time using scanning electron microscopy.
Results: The numbers of the adherent cells were increased with time and after 24 hrs, the highest number of attached cells were detected on SLA-Active disks (80,27%) than on the other groups (SLA 64% , Machine surface 48,57% and RBM surface 47,67%). The results of the cell spreading assay showed that there were no significant differences between SLA surfaces and SLA-Active surfaces after 24 hrs. However; the rates of the cell spreading of SLA-Active surfaces (40,38%) was higher than SLA surfaces (38,56%). There were significant differences between the SLA–Active and the two Machine and RBM surfaces.
Conclusion: Osteoblast cells were cultured and spread successfully on the SLA and SLA Active implant surfaces. The SLA-Active implant surface seems to be more effective for the initial interactions between the implant and bone tissue.