RNA ISOLATION AND DETECTION OF CELLULAR RNA QUANTITY OF SPERMATOZOA AND EMBRYOS PRIOR TO GENE EXPRESSION ANALYSES

Ozsait Selcuk, BİLGE; Coban, Neslihan; Sever Kaya, DİLEK; Bulgurcuoglu Kuran, SİBEL; Turkolmez, Selva; Dural, ÖZLEM

doi:10.26650/iuitfd.416666

RNA ISOLATION AND DETECTION OF CELLULAR RNA QUANTITY OF SPERMATOZOA AND EMBRYOS PRIOR TO GENE EXPRESSION ANALYSES

Ozsait Selcuk B. Ş., Coban N., Sever Kaya D., Bulgurcuoglu Kuran S., Turkolmez S., Dural O.

JOURNAL OF ISTANBUL FACULTY OF MEDICINE-ISTANBUL TIP FAKULTESI DERGISI, cilt.81, sa.4, ss.119-126, 2018 (ESCI, TRDizin)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 81 Sayı: 4
Basım Tarihi: 2018
Doi Numarası: 10.26650/iuitfd.416666
Dergi Adı: JOURNAL OF ISTANBUL FACULTY OF MEDICINE-ISTANBUL TIP FAKULTESI DERGISI
Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
Sayfa Sayıları: ss.119-126
İstanbul Üniversitesi Adresli: Evet

Özet

Objective: Biological samples that are analyzed in reproductive biology are generally rare, difficult to obtain, and a nonreplicable group of cells. Furthermore, investigating low numbers of cells requires modifications to the routine methods used in genetic analyses. The aim of our study was to improve RNA isolation methods for obtaining a sufficient amount of total RNA from spermatozoa and embryo samples for downstream gene expression analyses.