Kappa carrageenan/PEG-CuO nanoparticles as a multifunctional nanoplatform: digital colorimetric biosensor and anticancer drug nanocarrier


Karabatak A., DANIŞMAN KALINDEMİRTAŞ F., TAN E., Erdem-Kuruca S., KARAKUŞ S.

APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, vol.128, no.8, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 128 Issue: 8
  • Publication Date: 2022
  • Doi Number: 10.1007/s00339-022-05802-8
  • Journal Name: APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex
  • Keywords: Cisplatin, Multifunctional nanoparticle, Pancreatic cancer, Dopamine biosensor, EFFICIENT, DELIVERY, SENSOR, FLUORESCENCE, TOXICITY, ASSAY
  • Istanbul University Affiliated: Yes

Abstract

The development of green multifunctional nanoformulation has been given great attention with unique anticancer activity and ultra-sensitive sensing properties in biomedical applications. This study investigated the smartphone-integrated colorimetric dopamine sensing platform and the anticancer activity of Kappa Carrageenan/PEG-CuO nanoparticles (kappa CA/PEG-CuO NPs). The characterization of the prepared kappa CA/PEG-CuO NPs was conducted using scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), X-ray diffraction analysis (XRD), zeta-potential, Fourier transform infrared spectroscopy (FTIR), and ultraviolet-visible (UV-Vis) techniques. The surface characterization revealed that the obtained NPs had a spherical surface in the particle-size range of 5-10 nm. With a high coefficient of correlation (R-2 = 0.982), the digital colorimetric kappa CA/PEG-CuO NPs-based biosensor detected dopamine in a wide concentration range of 0.1-100 mu M and a low limit of detection (LOD) of 504 nM in 0.1 M phosphate buffer solution (PBS) (pH 7.4). In addition, the cytotoxicity of the prepared kappa CA/PEG-CuO NPs in living cells (HepG2 hepatocellular carcinoma), MIA PaCa-2 pancreatic cancer cells, and HUVEC (human umbilical vein endothelial cells) was investigated, which proved that kappa CA/PEG-CuO NPs exhibited high anticancer activity against MIA PaCa-2 pancreatic cancer cells. In conclusion, experimental results showed that multifunctional kappa CA/PEG-CuO NPs are both a promising biosensor for dopamine detection and an effective nanocarrier for pancreatic cancer therapy.