ABL gene kinase domain mutation scanning by denaturing high performance liquid chromatography sequencing method


Erbilgin Y., Catal S., Eskazan A. E. , Hatirnaz O., Soysal T., Ozbek U.

TURKISH JOURNAL OF HEMATOLOGY, vol.28, no.2, pp.97-102, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 2
  • Publication Date: 2011
  • Doi Number: 10.5152/tjh.2011.24
  • Journal Name: TURKISH JOURNAL OF HEMATOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.97-102
  • Istanbul University Affiliated: Yes

Abstract

Objective: Despite the efficacy of the BCR-ABL tyrosine kinase inhibitor imatinib, the development of resistance against imatinib has been observed. The most important mechanisms known to cause resistance are point mutations in the ABL tyrosine kinase and the ATP domain. This study describes the use of denaturing high performance liquid chromatography (dHPLC) as a method to screen for mutations of the ABL gene.