ABL gene kinase domain mutation scanning by denaturing high performance liquid chromatography sequencing method

Erbilgin Y., Catal S., Eskazan A. E., Hatirnaz O., Soysal T., Ozbek U.

TURKISH JOURNAL OF HEMATOLOGY, cilt.28, sa.2, ss.97-102, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 2
  • Basım Tarihi: 2011
  • Doi Numarası: 10.5152/tjh.2011.24
  • Dergi Adı: TURKISH JOURNAL OF HEMATOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.97-102
  • İstanbul Üniversitesi Adresli: Evet

Özet

Objective: Despite the efficacy of the BCR-ABL tyrosine kinase inhibitor imatinib, the development of resistance against imatinib has been observed. The most important mechanisms known to cause resistance are point mutations in the ABL tyrosine kinase and the ATP domain. This study describes the use of denaturing high performance liquid chromatography (dHPLC) as a method to screen for mutations of the ABL gene.