HLA DRB1 alleles, IFN-gamma and TGF-beta Gene Variants in childhood ALL patients

Oguz S. R., Ciftci H., Gokce M., Ogret Y., Kivanc D., Ozdilli K., ...Daha Fazla

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1515/tjb-2021-0202
  • Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, Food Science & Technology Abstracts, Directory of Open Access Journals
  • Anahtar Kelimeler: acute lymphoblastic leukemia, aGvHD, cytokine, genetic polymorphism, HLA, GRAFT-VERSUS-HOST, ACUTE GVHD, LEUKEMIA, DISEASE, RISK, TRANSPLANTATION, FREQUENCY, MARROW, PATHOPHYSIOLOGY, POLYMORPHISM
  • İstanbul Üniversitesi Adresli: Evet

Özet

Objectives Graft-versus-host disease (GvHD) is a complex clinical syndrome with organ dysfunction as a consequence of a severe immunological reaction mediated by mainly T cells after hematopoietic stem cell transplantation. Our aim is to evaluate the association of HLA-DRB1 alleles, IFN-gamma and TGF-beta gene variations, with childhood ALL (c-ALL) patients and with GvHD after transplantation. Methods This study included 30 high-risk c-ALL patients and 100 controls. HLA-DRB1 alleles were studied by the NGS method, and TGF-beta and IFN-gamma variations were studied by the PCR-RFLP method. Results The rates of HLA-DRB1*15 alleles and IFN-gamma CC genotype were significantly higher in c-ALL patients (p=0.004, p=0.036 respectively). Association of the HLA-DRB1*15 alleles with the TGF-beta TC genotype was found with a higher rate in the patient group (p=0.031). Association of the DRB1*04 allele with the IFN-gamma CC genotype was found with a higher rate in the patient group (p=0.028). Acute GvHD developed in eight of 19 patients who underwent transplantation. IFN-gamma CT was found to have a protective role in occurrence of aGvHD (p=0.044). Association of the DRB1*15 allele with IFN-gamma TT was found with a higher rate in a GvHD (p=0.050). Conclusions It is thought that polymorphism of HLA-DR15 and IFN-gamma CC may contribute to the development of c-ALL, while IFN-gamma CT might be protective for aGvHD.