Propagation and Growth Kinetics of Sheep Pox Vaccine Virus in MDBK Cells


Uzar S., TURAN N.

ACTA VETERINARIA EURASIA, vol.48, no.3, pp.177-182, 2022 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 48 Issue: 3
  • Publication Date: 2022
  • Doi Number: 10.5152/actavet.2022.21082
  • Journal Name: ACTA VETERINARIA EURASIA
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.177-182
  • Keywords: MDBK, propagation, sheep pox, virus, vaccine, LUMPY SKIN-DISEASE, EFFICACY, BOVINE
  • Istanbul University Affiliated: No

Abstract

Capripoxviruses include lumpy skin disease virus, sheep pox virus, and goat pox virus, which lead to economic impacts in ruminants. Live, attenuated commercial vaccines can be applied in cattle against lumpy skin disease virus because of cross-protection. Bakirkoy sheep pox vaccine is a live, attenuated vaccine used against lumpy skin disease virus in cattle in Turkey. Continuous cell lines are used in viral vaccine production. This study was performed to the propagation of vaccine virus in the Madin-Darby bovine kidney cells and determination of growth kinetics. At the seventh day post-inoculation, the cells became round and moved away from the surrounding cells and cytopathic effect formation started. After 80% cytopathic effect was formed on the tenth day post-inoculation, virus suspension was harvested and identified as sheep pox virus with real-time polymerase chain reaction analysis and serum neutralization test. The titre of the sheep pox-Madin-Darby bovine kidney vaccine virus was calculated as 10(5.75)/mL TCID50. The highest value was observed on the seventh day but the titre decreased on the ninth day while virus titre of supernatant was detected the highest on the seventh day post-infection and it decreased on the eighth day post-inoculation. The highest titre was reached in the third freezing and thawing and decreased in the fifth thaw. In conclusion, the Madin-Darby bovine kidney cells can be used in sheep pox vaccine production.