The effect of vitamin E treatment on selected immune and oxidative parameters in Kivircik ewes suffering from transport stress

Danyer E., BİLAL T., ALTINER A., AYTEKİN İ., ATALAY H.

JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION, vol.105, no.S1, pp.34-41, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 105 Issue: S1
  • Publication Date: 2021
  • Doi Number: 10.1111/jpn.13560
  • Journal Name: JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.34-41
  • Keywords: DL-alpha-tocopherol acetate, ewe, glutathione peroxidase, Kivircik, oxidative stress, transport stress
  • Istanbul University Affiliated: No

Abstract

The study aimed to investigate the effects of vitamin E injection for the prevention of transport stress on ewes. Kivircik ewes (2-3 years old, n = 24) were randomly separated into three groups; G1 (Control) and G2 treated with 14 ml. saline as the placebo, G3 treated with 2100 IU/ind. DL-alpha-tocopherol acetate prior to transport. G2 and G3 were transported at 80 km/h for 4 h on a truck. Serum samples were obtained before (T0) and after (T1) transport. Serum cortisol, catalase, IgG, ceruloplasmin, C-reactive protein, complement component 4, interleukin-1 beta, tumour necrosis factor-alpha, glutathione peroxidase (GPx), superoxide dismutase, malondialdehyde analyses performed by ELISA, and serum alpha-tocopherol concentrations were evaluated by HPLC-UV. Wilcoxon and Kruskal-Wallis tests were used for statistical assessments (p < 0.05). Alpha-tocopherol concentrations were founded 1.22 +/- 0.82, 0.27 +/- 0.14 and 0.14 +/- 0.07 mu mol/L, respectively, in G1, G2 and G3 at T1. Alpha-tocopherol concentration decreased significantly in G2 between T0 and T1. GPx concentrations were increased twofold in G2 and G3 between T0 and T1 (p < 0.01). As a result, G2 alpha-tocopherol concentrations decreased but, the stress and oxidative parameters tested in this study were not affected by treating 2100 IU/ind. DL-alpha-tocopherol acetate before transport.