Determination of levofloxacin by HPLC with fluorescence detection in human breast milk

Toker S. E., Kizilcay G., Sagirli O.

BIOANALYSIS, vol.13, pp.1063-1070, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 13
  • Publication Date: 2021
  • Doi Number: 10.4155/bio-2021-0058
  • Journal Name: BIOANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.1063-1070
  • Keywords: antibiotics, fluorescence detection, HPLC, human breast milk, levofloxacin, PERFORMANCE LIQUID-CHROMATOGRAPHY, PACKED SORBENT, HUMAN PLASMA, HUMAN URINE, CIPROFLOXACIN, GATIFLOXACIN, MOXIFLOXACIN, MICROEXTRACTION, TROVAFLOXACIN, SEPARATION
  • Istanbul University Affiliated: Yes

Abstract

Aim: A new HPLC method with fluorescence detection has been developed and validated for the determination of levofloxacin, one of the fluoroquinolone class antibiotics, in breast milk. Materials & methods: Chromatographic separation was carried out on a reversed phase C18 column with acetonitrile and 10 mM o-phosphoric acid (25:75,v/v) mobile phase composition. Moxifloxacin was used as internal standard and the peaks were detected by fluorescence detection. Results & conclusion: Calibration graph was found linearly within the range of 2.5-500 ng/ml. Limit of detection and limit of quantification were found to be 0.63 and 2.11 ng/ml, respectively. Mean absolute recovery was 96.18%. The developed method has been successfully applied to the determination of levofloxacin in human breast milk taken from two healthy volunteers.