Short Tandem Repeat-Polymerase Chain Reaction (STR-PCR) with Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) Method Using for Chimerism Analysis

Abatay-Sel F., Savran-Oguz F., Kalayoglu-Besisik S., Mastanzade M., Duvarci-Ogret Y., Yonal-Hindilerden I., ...Daha Fazla

CLINICAL LABORATORY, cilt.65, sa.9, ss.1697-1703, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65 Sayı: 9
  • Basım Tarihi: 2019
  • Doi Numarası: 10.7754/clin.lab.2019.190221
  • Dergi Adı: CLINICAL LABORATORY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1697-1703
  • Anahtar Kelimeler: allogeneic hematopoietic stem cell transplantation, chimerism analysis, multiplex STR-PCR, quantitative RT-PCR, MIXED HEMATOPOIETIC CHIMERISM, STEM-CELL TRANSPLANTATION, BONE-MARROW-TRANSPLANTATION, CHRONIC MYELOGENOUS LEUKEMIA, CAPILLARY-ELECTROPHORESIS, MICROSATELLITE MARKERS, PEDIATRIC-PATIENTS, AMPLIFICATION, ENGRAFTMENT, RELAPSE
  • İstanbul Üniversitesi Adresli: Evet

Özet

Background: Recently molecular chimerism analysis after allogeneic hematopoietic stem cell transplantation (AHSCT) has become more important. The use of quantitative chimerism methods aims to assess the kinetics of engraftment to determine graft rejection and failure or relapse of the underlying disease after AHSCT. An accurate and sensitive determination of chimerism status is mandatory after AHSCT. This study aimed to compare two chimerism methods: Multiplex Short Tandem Repeat-Polymerase Chain Reaction (STR-PCR) and quantitative Real Time-PCR (qRT-PCR).