Short Tandem Repeat-Polymerase Chain Reaction (STR-PCR) with Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) Method Using for Chimerism Analysis

Abatay-Sel F., Savran-Oguz F., Kalayoglu-Besisik S., Mastanzade M., Duvarci-Ogret Y., Yonal-Hindilerden I., ...More

CLINICAL LABORATORY, vol.65, no.9, pp.1697-1703, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 65 Issue: 9
  • Publication Date: 2019
  • Doi Number: 10.7754/clin.lab.2019.190221
  • Journal Name: CLINICAL LABORATORY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1697-1703
  • Keywords: allogeneic hematopoietic stem cell transplantation, chimerism analysis, multiplex STR-PCR, quantitative RT-PCR, MIXED HEMATOPOIETIC CHIMERISM, STEM-CELL TRANSPLANTATION, BONE-MARROW-TRANSPLANTATION, CHRONIC MYELOGENOUS LEUKEMIA, CAPILLARY-ELECTROPHORESIS, MICROSATELLITE MARKERS, PEDIATRIC-PATIENTS, AMPLIFICATION, ENGRAFTMENT, RELAPSE
  • Istanbul University Affiliated: Yes

Abstract

Background: Recently molecular chimerism analysis after allogeneic hematopoietic stem cell transplantation (AHSCT) has become more important. The use of quantitative chimerism methods aims to assess the kinetics of engraftment to determine graft rejection and failure or relapse of the underlying disease after AHSCT. An accurate and sensitive determination of chimerism status is mandatory after AHSCT. This study aimed to compare two chimerism methods: Multiplex Short Tandem Repeat-Polymerase Chain Reaction (STR-PCR) and quantitative Real Time-PCR (qRT-PCR).