Effects of Brassinosteroids on barley root growth, antioxidant system and cell division

Kartal G., TEMEL A., Gözükırmızı N.

XVI. FESPB Congress, Finland, 1 - 04 August 2008, pp.24

  • Publication Type: Conference Paper / Summary Text
  • Country: Finland
  • Page Numbers: pp.24
  • Istanbul University Affiliated: Yes

Abstract

Brassinolide (BR), which is the most biologically active of
the Brassinosteroids, was used to examine the potential effect of
hormone on barley (Hordeum vulgare L.) root germination, on the
changes in activities of antioxidative enzymes and cell division.
Barley seeds were germinated in between fi lter papers at dark 0.1,
0.5 and 1.0 ìM BR supplemented distilled water for 48 h with their
controls. BR application increased the root growth. The primary
root length almost doubled 1.62 ± 0.6 cm (in control) to 2.49 ± 0.8
cm (in 1.0 ìM BR treated materials) and obvious enlargements at
the root tips were observed. Superoxide dismutase activity signifi -
cantly increased at 1.0 ìM BR concentration when compared with
the control materials. However, no signifi cant changes in catalase
activity but decrease in peroxidase activity at the same concentration
was observed. Treated and untreated control group roots
were fi xed in 1:3 aceto-alcohol and aceto-orsein smear preparations
were made. For each experimental group, the percentage
of cells showing abnormalities were calculated. Roots treated
with BR showed the more abnormalities and mitotic activity. Data
obtained could be benefi cial for the understanding of BR effects on
root development.