An ultrasensitive tumor enriched flow-cytometric assay for detection of isolated tumor cells in bone marrow of patients with breast cancer

Cabioglu V., Igci A., Yildirim E., Aktas E., Bilgic S., Yavuz E., ...Daha Fazla

AMERICAN JOURNAL OF SURGERY, sa.5, ss.414-417, 2002 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0002-9610(02)01015-2
  • Dergi Adı: AMERICAN JOURNAL OF SURGERY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.414-417
  • Anahtar Kelimeler: breast, isolated tumor cells, immunomagnetic enrichment, flow-cytometry, LYMPH-NODES, BLOOD, MICROMETASTASIS
  • İstanbul Üniversitesi Adresli: Evet

Özet

Background: An ultrasensitive tumor enriched flow-cytometric assay was used to determine its feasibility in detection of isolated tumor cells (TTC) in bone marrow (BM) of patients with breast cancer. Methods: Epithelial cells were removed by magnetic microbeads conjugated with an anti-cytokeratin 7/8 monoclonal antibody to enrich tumor cells in BM samples. A specific gate for MCF-7 breast cancer cells (gate(MCF-7) (cells)) was also taken into consideration in addition to a gate including all enriched BM cells (gate(enriched BM cells)) in flow-cytometric analysis to enhance the specificity of the method. Results: Nineteen patients with stage. I/II were evaluated. Ten patients (53%) were found to have cytokeratin positive (CK+) cells according to the gate(enriched BM cells) whereas 6 patients (32%) had CK+ cells when the gate(MCF-7 cells) was taken into account. Conclusions: New strategies in nonmorphological ultrasensitive techniques might be useful to categorize patients with ITCs having different tumor morphology and characteristics. (C) 2002 Excerpta Medica Inc. All rights reserved.