As a result of advances in technology and then the emergence of using Internet in every step of life, web that provides access to the documents such as picture, audio, animation and text in Internet started to be used. At first, web consists of only visual and text pages that couldn’t enable to make user’s interaction. However, it is seen that not only access to information but also analyzing, configuring, sharing information and creating new information came into the prominence in 21st century. Changing needs and conditions has led to the changing web and the emergence of Web 2.0. The purpose of the study is to be examined faculty of education students' the usage condition of Web 2.0 tools in terms of various variables. This is a descriptive style study with a survey model that aims to present faculty of education students' the usage condition of Web 2.0 tools. The population of research composed of 359 students who study at first grade of Faculty of Education in Istanbul University in 2008-2009 academic year. These students were taken from eight different departments through random sampling for this research. The data collection used in this research was a questionnaire that is developed by researcher with 18 items. One-way ANOVA, t-test, was made to determine whether there was a significant different between averages according to the varieties about individual properties. It is seen in analyses which were practiced in line with this aim that faculty of education students' the usage condition of Web 2.0 tools differentiates in terms of departments, gender, high school which they graduate, the year of Internet and computer usage, weekly average duration of Internet and computer usage. It can be generally said that faculty of education students don't use Web 2.0 applications in the expected level. It is very important that pre-service teachers should learn how to use these tools as well as they should receive education of computer literacy in the faculty of education which trains teachers. The other suggestion is that these tools should be integrated with courses which pre-service teachers receive in the prevocational training. Different samples can be chosen or sample can be enlarged in the further researches. As comparison can be made between students of different faculties, analyses can be thoroughly made by being benefited from qualitative research method.