Antioxidant and anticholinesterase active constituents from Micromeria cilicica by radical-scavenging activity-guided fractionation

Ozturk M., Kolak U., Topcu G., Oksuz S., Choudhary M. I.

FOOD CHEMISTRY, vol.126, no.1, pp.31-38, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 126 Issue: 1
  • Publication Date: 2011
  • Doi Number: 10.1016/j.foodchem.2010.10.050
  • Journal Name: FOOD CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.31-38
  • Keywords: Lamiaceae, Micromeria cilicica, Antioxidant activity, Anticholinesterase activity, Piperitone 7-O-beta-D-glucoside, Isothymonin 4 '-methyl ether, ANTIMICROBIAL ACTIVITY, ESSENTIAL OIL, GLYCOSIDES, EXTRACT, MARKERS, WILD
  • Istanbul University Affiliated: Yes


From the acetone extract of Micromeria cilicica, two new (1 and 2) and five known (3-7) compounds were obtained through radical-scavenging activity guided isolation. Structures of the compounds were identified as piperitone 7-O-beta-D-glucoside (1), isothymonin 4'-methyl ether (2), sudachitin (3), isomucronulatol (4), rutin (5), ursolic acid (6) and saccharose (7), based on UV, 1D-and 2D-NMR and mass spectroscopic techniques. The antioxidant potentials of the extract and the isolated compounds were established by using three radical-scavenging assays, namely, DPPH. scavenging, O-2(center dot-) scavenging and ABTS(center dot+) scavenging, besides beta-carotene bleaching assay. Particularly, the acetone extract showed a strong inhibition of lipid peroxidation by beta-carotene bleaching assay, with a result close to that of (+)-catechin. Among the pure compounds, rutin (5) showed the strongest lipid peroxidation inhibition and antiradical activity while both sudachitin (3) and isomucronulatol (4) exhibited noticeable ABTS(center dot+) scavenging activity. The anticholinesterase activity of the compounds (1-7) was also determined. Against acetylcholinesterase, they exhibited weak inhibition while compounds 3, 4 and 6 exhibited moderate inhibition against butyrylcholinesterase. (C) 2010 Elsevier Ltd. All rights reserved.