Serological Investigation of Antibodies Induced by Smooth and Rough Brucella Lipopolysaccharide in Sheep
World's Veterinary Journal, cilt.16, sa.3, ss.900-908, 2026 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 16 Sayı: 3
- Basım Tarihi: 2026
- Doi Numarası: 10.54203/scil.2026.wvj82
- Dergi Adı: World's Veterinary Journal
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.900-908
- Anahtar Kelimeler: Brucellosis, Rough lipopolysaccharide, Sheep, Slaughterhouse, Smooth lipopolysaccharide
- İstanbul Üniversitesi Adresli: Evet
Özet
Different Brucella species, characterized by smooth and rough cell walls, are recognized as etiological agents of ovine brucellosis. While serological studies in adult and older sheep are common, those on the serological status of young animals are limited. The present study aimed to investigate brucellosis in sheep caused by smooth- and rough-walled Brucella species at two slaughterhouses in the Marmara Region of Turkey using serological methods. Three different analyses, including the rapid slide test, the tube agglutination test, and the indirect enzyme-linked immunosorbent assay (iELISA), each included smooth- and rough-structured Brucella antigens, resulting in a total of six separate tests. Blood sera were randomly obtained from 150 male sheep, approximately 1 year old and weighing about 70 kilograms, from the Merino, Kivircik, Lacauné, and Tahirova breeds. These sheep were brought to slaughterhouses from several provinces, including 25 from Tekirdağ, 45 from İstanbul, 43 from Kırklareli, 17 from Çanakkale, and 20 from Kocaeli. Using the rough iELISA, 15 positive cases (10%) were identified, of which eight were concurrently positive with the smooth iELISA. The low positivity rate and negative results from tube agglutination and rapid slide tests decreased the probability that the sheep had active brucellosis. Positive results for the same sample in both S-iELISA and R-iELISA tests may be linked to smooth LPSs, while positivity only in R-iELISA could be attributed to rough LPSs. Although no Brucella ovis or Brucella melitensis were found in the animals examined in the present study, the positive ELISA results suggested that brucellosis could have important epidemiological implications. Using two iELISA methods with smooth and rough LPS antigens concurrently enhanced the diagnostic sensitivity of indirect serological diagnosis of brucellosis.