Tetraphenylethylene-thiosemicarbazone based ultrafast, highly sensitive detection of hypochlorite in aqueous environments and dairy products


Erdemir E., Suna G., Gunduz S., Şahin M., Eglence-Bakir S., Karakus E.

ANALYTICA CHIMICA ACTA, vol.1218, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1218
  • Publication Date: 2022
  • Doi Number: 10.1016/j.aca.2022.340029
  • Journal Name: ANALYTICA CHIMICA ACTA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Tetraphenylethylene, Thiosemicarbazone, Fluorescent probe, Hypochlorite detection, Dairy products analysis, MYELOPEROXIDASE, PROBE, WATER
  • Istanbul University Affiliated: Yes

Abstract

We have designed and synthesised a novel fluorescent probe with a tetraphenylethylene (TPE) scaffold as an active fluorescent unit and thiosemicarbazide (TSC) group as a recognition unit. The probe, TPE-TSC, exhibited superior selectivity towards hypochlorite (ClO- ) with a low limit of detection (2.0 nM). It also demonstrated a turn-off response for a brief period (<30 s) via an oxidation reaction. Furthermore, high-resolution mass spectrometry (HRMS) revealed that TPE-TSC reacted with ClO- by forming a carboxylic acid moiety in nearly 100% aqueous environments. More significantly, the probe detected ClO- in disinfectant, spiked milk samples, and spiked water samples. In all, TPE-TSC proposes an optimistic approach precisely for the determining the quality of milk and water contaminated with ClO- and trace amounts of ClO- in disinfectants.