Determination of pethidine of abuse and relevant metabolite norpethidine in urine by ultra-performance liquid chromatography-tandem mass spectrometry

Kul A., Ozdemir M., Sağırlı A. O.

Journal of Pharmaceutical and Biomedical Analysis, cilt.186, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 186
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.jpba.2020.113320
  • Dergi Adı: Journal of Pharmaceutical and Biomedical Analysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chimica, EMBASE, International Pharmaceutical Abstracts, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Abuse of the drug, Method development and validation, Pethidine and norpethidine, Ultra-Performance liquid chromatography-tandem mass spectrometry, Urine samples, WHOLE-BLOOD, MS/MS METHOD, BODY-FLUIDS, LC-MS/MS, OPIOIDS, DRUGS, ANALGESICS, VALIDATION, PLASMA
  • İstanbul Üniversitesi Adresli: Evet

Özet

Pethidine is an opiate agonist used orally and parenterally. Pethidine-containing drugs abuse is frequently encountered on health workers and patients. The analysis methods used to determine the abuse of pethidine are important for forensic toxicology. Pethidine is metabolized to norpethidine by the liver. Therefore, the determination of pethidine and norpethidine in urine is one of the methods to determine the abuse of pethidine. In this study, we have developed a precise, simple and rapid ultra-performance liquid chromatography-tandem mass spectrometer method for the determination of pethidine and norpethidine simultaneously. The developed method was validated in terms of selectivity and linearity which was in the range of 9-1800 ng/mL for both pethidine and norpethidine. The intra-assay and inter-assay accuracy and precision were found within acceptable limits of the EMA guideline. Lower limits of quantitation were 9 ng/mL for both pethidine and norpethidine. The developed method was successfully applied for the determination of both analytes in the real samples. (C) 2020 Elsevier B.V. All rights reserved.