Akademik Veri Yönetim Sistemi
the 16th International Congress on the Zoogeography and Ecology of Greece and Adjacent Regions (ICZEGAR), Athens, Yunanistan, 3 - 07 Temmuz 2025, cilt.1, sa.1, ss.98, (Özet Bildiri)
The aim of this study is to conduct a genetic analysis of the Bulgarian minnow Phoxinus strandjae distributed in the Sapanca Basin (NW Türkiye) and to contribute to both the biodiversity data of Türkiye and the scientific literature by submitting the obtained data in GenBank. A total of 163 specimens of P. strandjae, representing 11 populations, were collected using a SAMUS-725G electrofishing device from four stations situated within the lotic systems of the Sapanca Basin between September 2023 and July 2024. The dorsal fin tissues were dissected under laboratory conditions from the collected fish specimens for DNA isolation. DNA extractions from the dissected tissue samples were performed using the Qiagen DNeasy Blood & Tissue Kit following the manufacturer’s protocol. The DNA barcode region (COI) and nuclear ISSR1 and ISSR3 loci were employed to assess the genetic variation among populations. Phylogenetic analyses were conducted using MEGA X, DnaSP 6 and Popgene 1.32. MEGA X was utilized to construct phylogenetic trees and calculate pairwise genetic distances. DnaSP 6 was employed to assess haplotype diversity, nucleotide diversity, and polymorphism indices, while Popgene 1.32 was used to estimate genetic differentiation parameters. The conducted genetic analyses provide a fundamental framework for understanding the population structure, genetic diversity, and evolutionary dynamics of P. strandjae in the Sapanca Basin. The present study effectively determined whether hydraulic barriers in the Sapanca Basin have led to genetic differentiation or caused a genetic bottleneck among the upstream and downstream populations of this species.
Keywords: Genetic diversity, DNA barcode, ISSR, freshwater fish, population differentiation
This study was supported by the TÜBİTAK-1001 (Project number 122Y318).