Effects of Different Transport Temperatures on in vitro Development of Queen Oocytes

Ozdas O. B., Baran A., Sandal A. I., Bakirer G., Tek C., Enginler S. O., ...More

JOURNAL OF ANIMAL AND VETERINARY ADVANCES, vol.11, no.7, pp.995-999, 2012 (SCI-Expanded) identifier identifier


Today, many species, especially feline are endangered. For this, domestic cats used as a model for in vitro culture studies. So, many investigators have problems transporting ovaries to their laboratories. In this study effects of different transport temperatures on in vitro maturation of feline oocytes were investigated. Ovaries were collected from 12 ovariectomised queens of 2-3 years old, four of which were at oestrus and eight at anoestrus. One ovary of each pair was brought to the laboratory in PBS at 4 degrees C and other one at 37 degrees C. Two main groups as oestrus and anoestrus were established and each were divided into further 2 subgroups as 4 and 37 degrees C. Oocytes were collected in TCM-medium and matured for 24 h under 5% CO2 at 38.5 degrees C, Matured oocytes were fertilized with fresh semen at a final concentration 1 x 10(6) mL(-1) in Sperm-Talp medium under 5% CO2 for 18-24 h. After removing cumulus oophorus cells and zygotes were divided into groups and in vitro cultured for 72 h in 100 mu L SOF medium under 5% CO2, 5% O-2 and 90% N-2 gas mixture. At the 48th h of incubation, the best cleavage was 44.4% (8/18) at 37 degrees C oestrus group and the lowest was 14% (7/50) in the oestrus group at 4 degrees C. These rates were 22.72% (15/66) and 28.57% (8/28), respectively for the anoestrus group. At the 72 h of culture in 37 degrees C oestrus group 7 embryos stayed at 4-8 blastomere stage and 1 embryo reached 16-32 blastomere stage. This result was significant when compared to the other groups (p<0.001). In 4 degrees C anoestrus group only 5 embryos have reached 4-8 blastomere and no significant difference among the results was observed. It is concluded that cat ovaries oestrus are better transported at 37 degrees C while anoestrus ovaries could be carried at 4 degrees C.