Brassinosteroids are steroidal plant hormones that influence varied growth and development processes such as germination of seeds, flowering, senescence and abscission. This study aimed to explore the effect of cotyledon growth by different epibrassinolid (epiBL) concentrations in excised red cabbage cotyledons. As an experimental material, the cotyledons of 8-day-old Brassica oleraceae seedlings, excised from the petiole were incubated in 3 nil distilled water (control group) and in epiBL of various concentrations (0. 001, 0. 1 and 10 mu M) for 3 days. Following incubation, peroxidase activity, total chlorophyll, soluble protein and anthocyanin contents were determined. The data indicate that the effect of epiBL on the growth of excised red cabbage cotyledons show difference related to concentration.