Chemical Compositions by Using LC-MS/MS and GC-MS and Biological Activities of Sedum sediforme (Jacq.) Pau

Ertas A., Boga M., Yilmaz M. A., Yesil Y., Hasimi N., Kaya M. S., ...Daha Fazla

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, cilt.62, sa.20, ss.4601-4609, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 62 Sayı: 20
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1021/jf500067q
  • Dergi Adı: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4601-4609
  • Anahtar Kelimeler: Sedum sediforme, phenolic content, essential oil, fatty acid, antioxidant, anticholinesterase, antimicrobial, quercetin, LC-MS/MS, GC-MS, ESSENTIAL-OIL COMPOSITION, ANTIOXIDANT PROPERTIES, ANTIMICROBIAL ACTIVITIES, FLAVONOL GLYCOSIDES, PROPOLIS, EXTRACTS, PLANTS, L.
  • İstanbul Üniversitesi Adresli: Evet

Özet

In this research, the chemical composition and biological activities of various extracts obtained from whole parts of Sedum sediforme (Jacq.) Pau were compared. The amounts of total phenolic and flavonoid components in crude extracts were determined by expression as pyrocatechol and quercetin equivalents, respectively. All of the extracts (petroleum ether, acetone, methanol, and water) obtained from S. sediforme showed strong antioxidant activity in four tested methods. Particularly, the IC50 values of the methanol extract, which was the richest in terms of total phenolic and flavonoid contents, were found to be lower than those of alpha-tocopherol and BHT in beta-carotene bleaching (9.78 +/- 0.06 mu g/mL), DPPH free radical scavenging (9.07 +/- 0.07 mu g/mL), and ABTS cation radical scavenging (5.87 +/- 0.03 mu g/mL) methods. Furthermore, the methanol extract of S. sediforme showed higher inhibition activity than galanthamine against acetyl- and butyryl-cholinesterase enzymes. Also, acetone and methanol extracts exhibited moderate antimicrobial activity against Candida albicans. The main constituents of fatty acid and essential oil were identified as palmitic acid (C16:0) (28.8%) and alpha-selinene (20.4%), respectively, by GC-MS. In the methanol extract of S. sediforme, quercetin, rutin, naringenin, and protocatechuic, p-coumaric, caffeic, and chlorogenic acids were detected and quantified by LC-MS/MS. Results of the current study showed that the methanol extract of S. sediforme may also be used as a food supplement.